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Figure 1


Fig. 1. Identification of coronin 3 as a GDP-Rab27a-interacting protein in pancreatic β cells. (A,B) Eluates from affinity columns were analyzed by silver staining (A) and immunoblotting with anti-coronin 3 antibody (B). (C) Immunoprecipitation of MIN6 extracts using anti-Rab27a antibody and immunoblotting using anti-Rab27a and anti-coronin 3 antibodies. 0.2% of the input protein was co-immunoprecipitated. (D,E) Mouse pancreata were double-stained using anti-insulin antibody and anti-Rab27a antibody (D) or anti-coronin 3 antibody (E). Scale bars, 20 µm.





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