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Fig. 1. Generation of REMI mutants exhibiting higher basal levels of GFP-PHcrac at the plasma membrane. (A) remi56 cells displayed more plasma membrane localization of GFP-PHcrac, which suggests higher PtdIns(3,4,5)P3 levels in the membrane of remi56. (B) Wild type and remi56 lines were tested to see whether they are able to form developmental territorial streams. remi56 lines failed to aggregate in submerged culture at the cell density of 2.5x10 cells/cm, whereas wild type cells aggregated normally.