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Figure 7


Fig. 7. Defects in sodC– cells were partially rescued by SodC but not by a SodC(H245R,H247Q) double-point mutation. (A) RT-PCR experiment with a primer set specific for SodC was used to detect the level of SodC transcript. A specific RT-PCR product was obtained from wild type, but not from sodC– cells as expected. Ig7 transcripts were used as a control. Similar levels of the transcripts were observed in sodC– cells expressing wild-type SodC and the SodC(H245R,H247Q) mutant under Actin-15 promoter. (B) sodC– cells expressing wild-type SodC and SodC(H245R,H247Q) mutant were challenged with a micropipette filled with 0.1 µM cAMP for 20 minutes. Twenty stacks of tracing images are shown with a 100 µm scale bar. Summary of the roundness (C) is shown.





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