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Figure 3


Fig. 3. The activity of DT on mouse embryonic fibroblasts. White bars indicate luciferase activity following transfection of pGL3-P luciferase reporter alone into the cells. Dark-grey bars show luciferase activity after transfection of the luciferase reporter plus pPGK-DTA at a molar ratio of 4:1. Light-grey bars show a co-transfection control, showing luciferase activity following transfection of the luciferase reporter plus pBluescript vector only, at a molar ratio of 4:1. Transfections into six different cell lines are shown, representing one wild-type (WT), two heterozygous (het) and three homozygous mutant (mut) genotypes. For each set of transfections the mean of the luciferase reporter alone is normalized to 100%. Error bars are ± s.d.





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