spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Effect of bound antibodies, GA and CPZ on ErbB2 localization in SK-BR-3 cells. (A) ErbB2 in fixed, permeabilized cells detected by immunofluorescence. (B) Cells were warmed for 2 hours after binding Fl-anti-ErbB2 before fixation. (C) Cells were treated with GA for 2 hours before detecting ErbB2 by immunofluorescence. (D,E) Cells were pre-treated for 45 minutes at 37°C with GA, with (D) or without (E) 12 µg/ml CPZ, before binding anti-ErBb2 antibodies (left, green) and Alexa-Fluor-594-Tf (middle, red) for 1 hour on ice and warming for 2 minutes with the same drugs. Cells were acid-washed and processed for immunofluorescence, detecting ErbB2 with the Alexa-Fluor-488 Zenon mouse IgG labeling kit. Merged images are shown on the right. Scale bar: 10 µm. (F,G) Internalization of biotinylated Tf (F) or biotinylated anti-ErbB2 antibodies (G) was measured by CELISA after treatment with GA (circles) or both GA and CPZ (squares). Values shown are the mean ± s.e.m. of three experiments.





Right arrow Return to article