spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Localization of internalized ErbB2, Rh-Tf, EGFR and clathrin. SK-BR-3 cells were pretreated with GA for 1 hour before binding Fl-anti-ErbB2 (A,C,E,F) or Fl-anti-EGFR (B,D,G) and warming for 5 minutes (with Rh-Tf in A,B and E) before acid washing and immunofluorescence analysis. (C,D) Clathrin heavy chain (CHC, red) was detected by immunofluorescence. (E-G) High-magnification views of the boxed regions in A,C and D, respectively. Right-hand panels in A-D and bottom panels in E-G show merged images. (A) Epifluorescence images. All other panels show maximum-intensity projections of deconvolved z-stacks. Scale bars: 10 µm (bar in B applies to B-D). (H) Quantification of colocalization of Rh-Tf with ErbB2 or EGFR in cells treated as in A,B, except that internalization was for 2 minutes.





Right arrow Return to article