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Figure 8


Fig. 8. ErbB2 is delivered to early endosomes after GA treatment. (A-D) SK-BR-3 cells were left untransfected (A,B), or were transiently transfected with GFP-Rab5 (C) or GFP–Rab5-Q79L (D), and then treated with GA for 2 hours (with Rh-Tf added for the last 30 minutes in A), fixed and permeabilized. Left panels: ErbB2, detected with polyclonal antibodies. Center panels: (A) Rh-Tf fluorescence; (B) endogenous EEA1; (C,D) GFP fluorescence. Right panels: merged images. (A) Deconvolved image from a z-stack; (B-D) epifluorescence images. Scale bars: 10 µm (A); 5 µm (D, applies to B-D).





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