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Figure 2


Fig. 2. RAB-11.1 and SYN-4 are required for exocytosis of cortical granules. The subcellular localization of CAV-1::GFP was determined in mock (A,B), rab-11.1(RNAi) (C,D), syn-4(RNAi) (E,F) and emb-27(RNAi) (G,H) animals by confocal laser microscopy. (A) In mock RNAi-treated animals, CAV-1::GFP gradually appears in ring-shaped structures (CAV-1 bodies/cortical granules, the inset) as oocytes grow. (B) The cortical granules are synchronously exocytosed in fertilized embryos and CAV-1::GFP targeted to the PM is then internalized for degradation within one-cell cycle. (C-F) RNAi of rab-11.1 or syn-4 causes accumulation of cortical granules around the nuclear envelope in oocytes (C,E, arrowheads) and inhibits fusion of cortical granules with the PM in embryos after fertilization (D,F). In the emb-27(RNAi) worm, the localization of CAV-1::GFP is normal in oocytes (G) but cortical granules remain in embryos (H). Oocytes and embryos are maturated from left to right. The numbers indicate the positions of oocytes and embryos from the spermatheca. The insets show 2x enlargements of the boxed area. Scale bars: 10 µm.





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