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Figure 4


Fig. 4. Dynamic behavior of GFP::RAB-11.1 during oocyte-embryo transition. (A,B) Subcellular localization of GFP::RAB-11.1 in wild-type oocytes. GFP::RAB-11.1 localizes to the cortical region in growing oocytes (–3 and –2 oocytes) but transiently accumulates on the ring-like structures in the ovulating oocyte (–1 oocyte). Arrowheads indicate Golgi-like punctate structures deeper in the cytoplasm. Middle (A) and top (B) focal planes are shown. (C) Subcellular localization of GFP::RAB-11.1 in wild-type embryos. A projected image of confocal z-stacks is shown. GFP::RAB-11.1 localizes on the ring-shaped structures in the early one-cell stage embryo (+1 embryo). (D) Subcellular localization of GFP::RAB-11.1 in emb-27(RNAi) embryos. GFP::RAB-11.1 stably localizes on the ring-shaped structures in +1 and +2 embryos arrested at the one-cell stage. Some GFP::RAB-11.1 localizes on large cytoplasmic foci in +3 embryo (asterisks). Insets in A-D show 2x enlargements of the boxed areas. (E-H) GFP::RAB-11.1 localizes on cortical granules in wild-type one-cell stage embryos. Embryos were dissected from animals expressing GFP::RAB-11.1 and stained sequentially with WGA-TRITC and an anti-GFP antibody. Enlarged images of the boxed area in E are shown in F-H. Arrowheads show colocalization of GFP::RAB-11.1 and WGA. Scale bars: 10 µm in A-E; 2.5 µm in H. SP, spermatheca.





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