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Figure 5


Fig. 5. Subcellular localization of SYN-4 and SNB-1 in oocytes and embryos. (A,B) The gonads of animals expressing CAV-1::GFP were stained with anti-SYN-4 (A) or anti-SNB-1 (B) antibodies. SYN-4 localizes mainly on the PM but a part of SYN-4 is detected on cortical granules (arrowheads in A). SNB-1 localizes on the cortical granules (arrowheads in B) and small cytoplasmic vesicles. (C) The gonads of wild-type animals were stained with anti-SNB-1 antibody and WGA-TRITC. SNB-1 localizes to cortical granules labeled with WGA (arrowheads). (D,E) Subcellular localization of GFP::SNB-1 in the germline. In oocytes, GFP::SNB-1 localizes to ring-shaped structures (D). Unlike CAV-1::GFP, GFP::SNB-1 is not degraded in embryos and the GFP signal is stably detected in developing embryos (E). The insets show 2x enlargements of the boxed areas. Scale bars: 10 µm.





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