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Figure 3


Fig. 3. Nuclear rotation includes ER closely associated with the nuclear membrane. (A) Cells transfected with dsRed2-ER were grown to confluency and wounded. The top panel shows a nucleus rotating clockwise during migration. White arrowhead indicates the position of a nucleolus during rotation. The bottom panel shows the morphology of the ER during the same timeframe. Some small fragments of the ER that are closely opposed to the nucleus rotate with it (black arrowhead, see also supplementary material Movie 3). Peripheral ER shows dynamic remodeling during the rotation. (B) Tracks of the paths of a single nucleolus and an ER particle within the cell shown in A during nuclear rotation over 45 minutes. Both follow the same circular path, indicating that they are both rotating in the same manner (see also supplementary material Movie 3). Time is minutes:seconds. Scale bar, 10 µm.





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