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Figure 4


Fig. 4. Nuclear rotations are not coupled to centrosome rotation. (A) Exogenous Centrin2 closely associates with endogenous {gamma} tubulin in puncta capable of MT nucleation, supporting its functional role as a centrosome marker. Cells transfected with dsRed1-Centrin2 (red) were subjected to nocodazole treatment. After a 5-minute wash, cells were fixed and stained for {gamma} tubulin (green) and {alpha} tubulin (blue). (B) Tracks of a single nucleolus (red) and a centrosome (blue) within the cell shown in C during nuclear rotation over 45 minutes. The cell is migrating towards the top of the graph, and the nucleus rotates while the centrosome remains between the nucleus and the leading edge. (C) Cells transfected with dsRed1-Centrin2 (red), a centrosomal marker, were grown to confluency and wounded. The arrowhead indicates a centrosome that stays positioned between the nucleus and the leading edge while the nucleus rotates clockwise (black arrow, also see supplementary material Movie 5). Time is minutes:seconds. Scale bars, 10 µm.





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