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Figure 2


Fig. 2. The requirement for WASp1 during the primitive macrophage response to a laser wound. (A,B) Whole-mount views of 22-hpf Tg(fli1a:EGFP) transgenic fish with the typical distribution of primitive macrophages (green cells) over the yolk (enclosed by the white broken line in the inset) at 0 minutes (left) and 60 minutes (right) post-wounding of control (A) and WASp1-morphant (B) embryos (wound centre indicated by an asterisk; see supplementary material Movies 1 and 2). (C) A graphic illustration of the number (n, y-axis indicates number of cells) of macrophages recruited to control versus morphant wounds and the mean speed (v, y-axis indicates mean speed in µm/minute) of these cells. (D,E) Illustrate two typical cell morphologies each of control (D) versus morphant (E) macrophages en route to the wound. Scale bars: A, 20 µm (inset, 50 µm); D,E, 4 µm.





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