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Fig. 6. Identification of WASp1 mutants by TILLING. (A) Schematic to illustrate the sites of the two mutations in the wasp1 gene identified from our TILLING screen. (B,C) Graphic illustrations of the number of neutrophils drawn to 90-minute fin wounds that were made to 3-dpf larvae with the genotypes +/+ (wild type), +/– (heterozygote) and –/– (mutant) derived from +/– x +/– crosses of our exon-2 (B) or exon-10 (C)-mutant lines. Amino acids remaining post stop codon are highlighted in red. (D) Illustrates fluorescent immunostaining of typical wild-type, heterozygote and WASp1-mutant wounds at 3 hours post-wounding to indicate the differential extent of leukocyte recruitment; the broken white line indicates the ventral margin of the vein and the asterisk marks the centre of the wound. (E) Blood smears from 1-month-old WASp1-mutant (exon 10) fish versus control sibs to illustrate morphologies of erythrocytes (e), thrombocytes (t), neutrophils (n) and lymphocytes (l). Scale bars: D, 15 µm; E, 4 µm.

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