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Fig. 2. Defective processing of Nedd8-CFP fusions in DEN1^null mutants. Western blot using larval lysates expressing myc-mNedd8 (lane 2), myc-Nedd8GG-CFP (lanes 3, 4) or myc-Nedd8AA-CFP (lanes 5, 6) transgene by tubP-GAL4 in wild-type or DEN1^null background probed with antibodies against Myc. The protein level of Myc-Nedd8GG-CFP at 40 kDa is more reduced in wild type compared with DEN1^null (lanes 3, 4, arrow on left). However, Myc-Nedd8AA-CFP is expressed at identical levels in both wild type and DEN1^null (lanes 5, 6). The product from the processing of Myc-Nedd8GG-CFP runs at 11 kDa with a mobility identical to Myc-mNedd8 (lane 2, arrowhead), but slightly slower than a non-specific proteolytic product in lanes 4-6 (asterisk). Lane 1 is wild-type control without transgene expression and the bottom panel shows ${alpha}$ -Tub expression.

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