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Fig. 5. Ectopic neddylated proteins in DEN1^null. (A) Expression of Myc-mNedd8-conjugated proteins was induced in DEN1^null, as shown in western blot using the anti-Myc antibody for larval extracts prepared from tubP-GAL4;UAS-myc-mNedd8 in the wild type (lane 1) or DEN1^null (lane 2) genetic background. (B) In western blot to detect endogenous neddylated proteins using anti-Nedd8 antibodies, ectopic neddylated proteins appear in DEN1^null (lane 2) when compared with wild type (lane 1), and disappears in DEN1^null;APP-BP1^EX62 (lane 4), which displays a similar Nedd8 pattern to APP-BP1^EX62 (lane 3). (C) Distinct neddylation patterns in DEN1^null and CSN5^null mutants in western blot using anti-Nedd8 antibodies for larval extracts prepared from wild type (lane 1), DEN1^null (lane 2), CSN5^null (lane 3) and DEN1^null;CSN5^null (lane 4).

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