spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. The cyclin E-interaction domain within MCM5. (A) HA-tagged MCM5 cyclin E-interaction fragments. The indicated HA-tagged MCM5 fragments were radiolabeled with [35S]methionine in an in vitro transcription-translation system and subjected to pulldown analysis as in Fig. 1B with GST or GST–cyclin E. Right: an autoradiograph is shown. Left: a Coomassie-stained gel of 25% of GST and GST cyclin E. (B) GFP-tagged MCM5 cyclin E-interaction fragments. The indicated GFP-tagged fragments were radiolabeled and analyzed by GST pulldown analysis as in A. Right: autoradiograph of pulldown. Left: Coomassie-stained gel of 25% of GST and GST proteins.





Right arrow Return to article