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Figure 5


Fig. 5. Effect of PACT–cyclin E expression. (A) Localization of PACT-domain-fused cyclin E. The indicated 6Myc-tagged PACT–cyclin E fusion proteins were expressed by transient transfection in CHO-K1 cells, methanol-fixed, and analyzed by immunofluorescence for {gamma}-tubulin and Myc–cyclin E. Arrowheads mark the centrosome. Inset: enlarged image of merged centrosomal area. Scale bars: 5 µm. (B) MCM5 centrosomal localization relies on an interaction with cyclin E. Endogenous MCM5 centrosomal localization was analyzed as in Fig. 4A in CHO-K1 cells co-expressing either GFP or GFP-CLS along with the indicated Myc-tagged PACT-fused cyclin E fusion constructs. Over 500 cells were analyzed for each condition in each experiment. Error bars indicate mean ± s.e.m. (n 3).





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