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Figure 6


Fig. 6. Expression of MCM5 but not MCM2 inhibits centrosome duplication. (A) Effect of MCM5 on centrosome number. HU-arrested CHO-K1 cells were transiently transfected with full-length HA-tagged MCM5. The number of {gamma}-tubulin-staining foci (centrosomes) was monitored by immunofluorescence in HA-expressing (white bars) and non-expressing (control) cells (black bars) 24 hours after transfection. (B) Statistical analysis of the effect of MCM5 on centrosome duplication. Several experiments similar to the one in A were performed with the indicated constructs. The bar indicates mean ± s.e.m. (n 3). Over 150 cells were counted for each condition in each experiment.





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