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Fig. 7. Expression of MCM5 does not displace endogenous cyclin E from centrosomes. CHO-K1 cells transiently transfected with full-length HA-tagged MCM5 were methanol-fixed after 24 hours and analyzed by immunofluorescence for the localization of endogenous cyclin E. Arrowheads mark the centrosome. Inset: enlarged image of the centrosomal area. Scale bar: 5 µm.