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Figure 3


Fig. 3. The RRR ER-retention/retrieval signal reduces surface expression of the CD8{alpha} chimeras. COS-7 cells were transfected with plasmids expressing Myc-CD8{alpha}, Myc-CD8{alpha}-KKTN, or with a series of constructs with the C-terminus of CD8{alpha} linked to 2L or to the various segments of 1L of Nav1.8. Non-permeabilized and permeabilized immunofluorescence labeling were carried out with antibodies against Myc. The images are representative of at least three independent experiments. Scale bar: 10 µm.





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