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Fig. 5. The C-terminus of the β3 subunit masks the RRR ER-retention/retrieval signal. (A) COS-7 cells were co-transfected with plasmids expressing Myc-CD8 ${alpha}$ -1L-1-3 and β1-GFP or β3-GFP, and subjected to cell-surface biotinylation/immunoblotting. Representative immunoblot and quantitative analyses are shown. Actin served as an internal control for protein loading. Data were plotted as a percentage of controls. ^*P<0.05 versus cells transfected with Myc-CD8 ${alpha}$ -1L-1-3 only (n=3). (B) COS-7 cells were co-transfected with plasmids expressing CD8β-GFP and Myc-CD8 ${alpha}$ , CD8β-β3C-GFP and Myc-CD8 ${alpha}$ -1L-1, CD8β-GFP and Myc-CD8 ${alpha}$ -1L-1, or CD8β-β3C-GFP and Myc-CD8 ${alpha}$ -KKTN. Cells were immunofluorescently labeled with antibody against Myc. The images are representative of at least three independent experiments. Scale bar: 10 µm. (C,D) COS-7 cells were co-transfected with CD8β-GFP or CD8β-β3C-GFP plus Myc-CD8 ${alpha}$ -1L-1, Myc-CD8 ${alpha}$ -1L-1-1, Myc-CD8 ${alpha}$ -1L-1-2 or Myc-CD8 ${alpha}$ -1L-1-3, and subjected to cell-surface biotinylation/immunoblotting. ^*P<0.05 versus cells co-transfected with CD8β-GFP (n=3).

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