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Figure 6


Fig. 6. The C-terminus of the β3 subunit interacts with segments that contain the RRR motif and promotes their surface expression. The C-terminus of the β3 subunit with the transmembrane domain (TM+β3C-GFP) was co-transfected with Myc-CD8{alpha}-1L-1, Myc-CD8{alpha}-1L-1-2, Myc-CD8{alpha}-1L-1-3 or Myc-CD8{alpha}-1L-1-3m in COS-7 cells. (A,C) Proteins were immunoprecipitated with GFP-specific antibody and cell lysates subjected to immunoblotting with antibodies against Myc or GFP as indicated. All experiments were repeated three times. (B,D-F) Transfected cells were subjected to cell-surface biotinylation/immunoblotting. Representative immunoblots are shown. Actin served as an internal control for protein loading. Quantitative data were plotted as a percentage of controls. *P<0.05 versus the control cells or the cells indicated (n=3).





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