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Fig. 7. HuD, but not IMP1, colocalizes with Dcp1a in HEK293 cells. (A) GFP-HuD construct (a) was transfected into HEK293 cells, followed by immunostaining with anti-Dcp1a antibody (b). Colocalization in P-bodies is indicated with white arrows (c). GFP-IMP1 construct was transfected into HEK293 cells (d), followed by immunostaining with anti-Dcp1a antibody (e). P-bodies are indicated with a yellow arrow (f). (B) Lysates of HEK293 cells transfected with GFP-Dcp1a were incubated with GST beads coupled to GST-tagged HuD protein.