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Figure 9


Fig. 9. Endogenous colocalization of HuD and Dcp1a in growth-cone-like structures of differentiated PC12 cells. PC12 cells were neuronally differentiated by incubation with 50 ng/ml NGF for 4 days. Cells were then fixed and immunostained using antibodies against HuD and Dcp1a. Fluorescein isothiocyanate (FITC)- and Cy3-conjugated secondary antibodies were used to label (A) Dcp1a and (B) HuD, respectively. (C) Merged image of both Dcp1a and HuD. Arrows indicate colocalization of both proteins. (D) Phase image of differentiated PC12 cells.





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