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Figure 2


Fig. 2. ATPe-induced ethidium uptake in HEK-P2X7 cells and in macrophages. Fluorescence images of cells incubated for 15 minutes at 37°C in the presence (B,D,F,G) or not (A,C,E) of 5 mM ATP. Ethidium bromide (10 µM) was added in all preparations. (A,B) Macrophages; (C,D) HEK-293 cells; (E,F) HEK-P2X7 cells. (G) HEK-P2X7 cells pre-incubated for 2 hours with 300 µM oxATP before the addition of ATP. Each result is representative of at least three independent experiments. Bar, 100 µm.





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