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Fig. 3. Large non-selective channels observed in macrophages but not in HEK-P2X₇ cells correlates with ATP_e-induced anion uptake. Cell-attached recordings (A-C) and dye uptake assays (D) were made in macrophages (A,C,D) and in HEK-P2X₇ cells (B). For patch-clamping experiments, cells were kept at 37°C and recordings were performed with a holding potential of –40 mV. ATP was applied manually with a micropipette (1 mM final concentration). Results are representative of at least ten independent experiments. (A) Cell-attached recordings of macrophages. Upper trace: normal extracellular solution. Lower trace: divalent-free extracellular solution. (B) Cell-attached recording of a HEK-P2X₇ cell. (C) I-V curves of the macrophage channels obtained in the same conditions as in the upper trace of A. (D) ATP_e-induced uptake of ethidium and LY in macrophages using the same solutions as in A. Data in D was obtained by fluorescence microscopy as described in Materials and Methods. The values were normalized by taking the specific fluorescence, obtained in normal solution in the presence of ATP, as 100%. Data points and bars represent the mean ± s.d. of at least four independent experiments.

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