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Fig. 6. The PCNA-binding domain of p21 inhibits the PCNA-pol ${eta}$ interaction after UV irradiation. (A) U2OS cells transfected with GFP-pol ${eta}$ and the indicated plasmids were UV irradiated. Four hours later, Triton-soluble (extractable, E) and Triton-insoluble (non-extractable, NE) fractions were collected and p21, PCNA and pol ${eta}$ distribution in both fractions was determined using specific antibodies. (B) U2OS cells transfected with GFP-pol ${eta}$ and the indicated plasmids were UV irradiated. Four hours later, the chromatin-bound fraction was cross-linked, sonicated and PCNA was immunoprecipitated (IP) as described in Materials and Methods. PCNA, GFP-pol ${eta}$ , pol ${delta}$ and p21 were detected utilizing specific antibodies. The left-hand set of panels shows PCNA IP, whereas that on the right shows an aliquot of the chromatin-bound fraction used for the PCNA IPs (INPUTS). (C) HCT116 p21^+/+ and p21^–/– cells (1x10⁶) were UV irradiated and treated as in B at the indicated time points. After PCNA immunoprecipitation, endogenous pol ${eta}$ , PCNA, pol ${delta}$ and p21 were detected utilizing specific antibodies. Note that high exposures of the blot to film were necessary to detect p21 in the IPs shown in C.

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