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First published online September 17, 2008

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Double the fun for MCM5?

Centrosomal duplication is necessary for the equal segregation of chromosomal DNA in mitosis, but this process must be tightly controlled – the over-duplication of centrosomes is commonly seen in cancerous cells and might contribute to aneuploidy. Cyclin E and its target, cyclin-dependent kinase 2 (Cdk2), which are key regulators of the G1-S transition of the cell cycle, are thought to control both centrosomal duplication and DNA replication, but other key players remain to be identified. On page 3224, Rebecca Ferguson and James Maller demonstrate a role for the centrosome-associated protein MCM5 (which is involved in the initiation of DNA replication) in the control of centrosomal duplication. The authors show that MCM5 interacts specifically with cyclin E independently of Cdk2, and that this interaction is necessary for the centrosomal localisation of MCM5. Moreover, the centrosomal localisation sequence (CLS) of cyclin E mediates its interaction with MCM5; the authors also identify the domain of MCM5 that interacts with the CLS. Importantly, the expression of MCM5 (but not the related protein MCM2) in CHO cells inhibits centrosome duplication. MCM5 might, therefore, inhibit the over-duplication of centrosomes and could represent a class of proteins that regulates both DNA replication and centrosome duplication.

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Related articles in JCS:

Cyclin E-dependent localization of MCM5 regulates centrosome duplication

Rebecca L. Ferguson and James L. Maller
JCS 2008 121: 3224-3232. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?