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Figure 2


Fig. 2. Inhibition of don3-as cells with NA-PP1 results in a reversible cytokinesis arrest. (A) In the presence of 1 µM NA-PP1 don3-as cells form large cell clusters. Scale bar, 10 µm. (B) don3-as cells were labelled with NHC-LS-biotin and the activity of Don3-as was inhibited with 1 µM NA-PP1. After 5 hours cells were washed and further incubated without inhibitor. Fluorescence and DIC images were taken at the time-points indicated. Scale bars, 10 µm. Diagrams indicate the abundance of single budding cells (1-2 cells) and cell clusters consisting of three or more cells in percent (n=200) (>3 cells).





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