spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Effect of protein kinase inhibitors on TLR-induced production of intracellular and extracellular TNF{alpha} in murine macrophages. (A) RAW cells were pre-treated for 60 minutes with or without 2 µM PD 184352 (PD) and then either left untreated or were stimulated for 4 hours with 100 ng/ml LPS. The cell culture supernatants were collected and the levels of TNF{alpha} analysed using the beadlyte system (histogram). The cell lysates (30 µg protein) were subjected to SDS-PAGE and, after transfer to nitrocellulose, the membrane was probed with a murine anti-TNF{alpha}-specific antibody or an anti-actin antibody (lower panel). (B) As in A, except that BMDM were used instead of RAW cells. (C) As in A, except that primary human monocytes-macrophages were used instead of RAW cells and 10 µM U0126 (U0) was used as an additional MKK1 inhibitor. (D) As in A, except that cells were stimulated for 4 hours with 100 ng/ml FSL1. The results are shown as mean±s.e.m. for three (A,B) or two (C,D) separate experiments.





Right arrow Return to article