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Figure 3


Fig. 3. Dynactin activity is required at least for EMS spindle rotation. Multi-photon time series of embryos expressing tubulin::GFP in (A) wild type (WT) and (B) dnc-1(or404ts) embryos treated with partial dnc-1 RNAi (see Materials and Methods) and imaged at permissive temperature showed normal EMS spindle rotation; (C) dnc-1(or404ts) embryos treated with partial dnc-1 RNAi and imaged at restrictive temperature showed EMS set up its metaphase spindle perpendicular to the A-P axis (this embryo shows a ventral aspect). Scale bar: 10 µm.





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