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Figure 3


Fig. 3. Melanogenic gene expression in purified primary wild-type and Nf1+/– melanocytes. FACS-separated Kit+CD45– wild-type and Nf1+/– melanocytes were plated at 1 x104 cells per well in individual wells of a 24-well plate and cultured in the presence of TPA, bFGF and dbcAMP for 10 days. Growth factors were withdrawn 72 hours prior to RNA isolation. The relative gene expression levels of (A) Tyr, (B) Tyrp1, (C) Dct and (D) Mitf were measured by real time RT-PCR. Results represent the normalized mean ± standard deviation of five independent measurements. P<0.05 for Nf1+/– versus wild-type melanocytes by Student's paired t-test.





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