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Figure 4


Fig. 4. feSEM and TEM of lamin B2-induced intranuclear membrane arrays. FLAG-tagged lamin B2 was expressed by nuclear injection of plasmid DNA. (A) Nuclear envelope of an oocyte overexpressing lamin B2, shown at low magnification. (B) Membrane arrays covered by irregular wavy lamin B2 filaments. (C) A more-ordered package of lamin B2 filaments on a membrane array. Note the more-or-less parallel alignment of B2 filaments. (D) TEM section of an isolated oocyte nucleus expressing lamin B2. (E,F) Immuno-feSEM detection of lamin B2 on membrane arrays using the mAb L7-8C6 that is specific for Xenopus lamin B2 (E) and antibodies against the FLAG epitope (F). The immuno-gold labels are indicated by gold spots in E and F.





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