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Figure 6


Fig. 6. FeSEM of isolated nuclear envelopes from oocytes expressing high levels of lamin A after injection of 1.5 ng of plasmid DNA. (A,B) Lamin A filaments that are arranged in layered bundles are indicated by small arrows; they surround, but appear not to interact with, structures that are consistent with NPC baskets. In general, the distal basket ring is visible (large arrows), and sometimes basket filaments can be seen (arrowheads). Oocytes expressing lamin A were also analysed by TEM thin sections and compared with controls. (C,D) The lamina is hardly discernible in (C) controls but forms a thick electron-dense layer in (D) oocytes expressing lamin A, which leaves the NPCs clear (arrows).





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