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Figure 1


Fig. 1. WNT3a stimulates activation of JNK and the AP-1–luciferase reporter in F9 cells. (A) Confluent wild-type F9 clones (F9) or F9 cells transfected with pCDNA3.1 alone (pcDNA) or F9 cells stably expressing rat Frizzled-1 (rFZ1) were treated with purified WNT3a for 0 to 60 minutes and JNK activation was measured by immunoblotting total lysates with phospho-specific antibodies against Jun. (B) rFZ1-expressing cells were transfected with 30 ng of AP-1–luciferase reporter plasmid in a 12-well plate for 24 hours, followed by 24 hours of serum starvation. The cells then were treated with or without WNT3a for 7 hours and luciferase gene reporter assays were performed, as described in the Materials and Methods. The upper panel displays the mean values±s.e.m. obtained from three independent experiments; the lower panel displays corresponding representative blots. For the AP-1-luciferase assay, the data represent the mean values±s.e.m. obtained from three independent replicate experiments. *P<0.05; **P<0.01 versus the time `zero' control.





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