spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Activation of the JNK pathway by WNT3a is not obligate for formation of primitive endoderm. F9 cells stably expressing rFZ1 were treated with a JNK inhibitor (0.4 µM SP600125) or transiently transfected with DN-RhoA or DN-MEKK 1 before stimulation with WNT3a for 4 days. Subsequently, the cells were prepared for immunocytochemistry and stained with a monoclonal antibody against the cytokeratin endo A (TROMA-1), a marker protein for primitive endoderm. Alexa-Fluor-488-conjugated secondary antibodies were employed together with indirect epifluorescence to detect the immune complexes. Typical phase-contrast images (PC) and the indirect immunofluorescence images (IIF) are shown from a single experiment, representative of three independent experiments.





Right arrow Return to article