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Fig. 1. Myofibroblasts and fibroblasts exhibit distinct periodic Ca²⁺_[i] oscillations. Spontaneous Ca²⁺_[i] oscillations in cultures of myofibroblasts (A-D) and of fibroblasts (E-H) (see also supplementary material Movie 1) were compared between contacting (continuous lines) and isolated cells (dotted lines). The Em₃₄₀/Em₃₈₀ fluorescence ratios of Fura-2-loaded cells were recorded every 3-5 seconds over regions of interest including the entire cell (A and E show Em₃₈₀ fluorescence image) and are plotted against time (B,F). The dominant periods of spontaneous Ca²⁺_[i] oscillations were determined by Fast Fourier Transform (Supplementary material Fig. S1A) of each profile and summarised in histograms for myofibroblasts (C, n_exp=25, n=193) and for fibroblasts (G, n_exp=18, n=184). (D,H) The dominant periods of Ca²⁺_[i] oscillations were separately analysed for contacting cell (blue histogram fit, myofibroblasts, n=97; fibroblasts, n=83) and isolated cells (red histogram fit, myofibroblasts, n=96; fibroblasts, n=101). This revealed no influence of the physical cell contact on the mean oscillation period within each group (Table 1). Scale bar: 50 µm.

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