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Figure 7


Fig. 7. Effect of Snail on expression of endothelial and mural markers in MESECs. (A-D) Levels of expression of claudin 5 (A), SMA (B), SM22{alpha} (C) and calponin (D) in MESECs transfected with Snail siRNA or scrambled sequence as a negative control (NTC), and cultured in the absence (–) or presence of TGFβ2 (Tβ) were analyzed by quantitative real-time RT-PCR. Error bars indicate s.d. (E) Protein levels of claudin 5 (top), SMA (middle) and {alpha}-tubulin (bottom) were examined by immunoblotting of total lysates of the MESECs described in A-D.





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