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Figure 8


Fig. 8. Specificity, time and spatial dependency of the βV spectrin–prestin association. (A) In the presence of OC lysates, only SLC26A5-C (prestin-C), but not SLC26A4-C (pendrin-C), SLC26A6-C or SLC26A7-C, binds to GST-tagged βVR29. (B) This interaction cannot be detected when prestin-FL lacks either the STAS domain (Del632) or the last 35 amino acid residues (Del709). (C) In the presence of protein extracts derived from P0 organs of Corti (a stage at which OHC electromotility is not yet present), prestin does not associate with GST-tagged βVR29. The interaction can be seen only when lysates from P4, P8 or P35 organs of Corti are used. (D) βV spectrin–prestin interaction in the presence of different mouse tissue lysates. An interaction cannot be detected when using lysates derived from the brain, liver or HEK293 cells. Vest, vestibule (balance organ). The same amounts of proteins were used at each developmental stage, as shown by western blot analysis of the lysates with an anti-actin antibody (C,D).





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