JCS031872 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Evaluation of the mRNA levels of neuroserpin (NS) in cerebral cortex of male and female C57Bl6/J mice. Wild-type and AMHR-II-deficient mice (heterozygotes and homozygotes) were compared in both males (filled marks) and females (open marks). No significant difference was observed (Kruskall-Wallis test).

• Supplemental Figure S2 -

  Fig. S2. Localization of AMHR-II and BMPR-II in testis and cortex. Double immunostaining of the adult mouse testis (A,C,E) and cortex (B,D,F) with goat polyclonal anti-BMPR-II and rabbit polyclonal anti-AMHR-II antibodies showing that primary antibodies recognized different antigens. In the testis, the immunoreactivity for AMHR-II TRITC in A is present in the seminiferous tubes while BMPR-II immunoreactivity is undetectable (C). In the cortex, both immunoreactivities are detected without any colocalization as shown by the absence of yellow labelling on the merged picture (F). The immunoreactivity for AMHR-II is distributed across the parenchyma and associated with capillaries while the immunoreactivity for BMPR-II is associated with the plasma membrane of a neuronal somata (arrow in D, F). The nuclei are counterstained with DAPI (blue; A-F). Scale bars represent 20 µm.

• Supplemental Figure S3 -

  Fig. S3. AMHR-II is associated with nerve fibers in white matter tracts. Immunohistochemistry for AMHR-II demonstrating the association of AMHR-II with nerve fibers in white matter tracts. In the cerebellum (A), the immunoreactivity for AMHR-II is present along nerve fibers of the white matter tracts (wm). Furthermore, it is detected in the parenchyma of the granular cell layer (gl) and associated with the vascularization. In the corpus callosum (B), the immunoreactivity for AMHR-II appears to be clearly associated with nerve fibers (arrowheads) and capillaries (arrows). The nuclei are counterstained with DAPI (blue) (A, B). cc, corpus callosum. Scale bars represent 50 µm for A and 20 µm for B.