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Figure 1


Fig. 1. Analysis of mitochondria in wild-type, loss-of-function ced-9(n2812lf); ced-3(n717lf) and gain-of-function ced-9(n1950gf) animals. (A-C) Mitochondrial morphology in wild-type, loss-of-function ced-9(n2812lf); ced-3(n717lf) and gain-of-function ced-9(n1950sd) genetic backgrounds was examined by creating multiple independently generated transgenic lines that expressed GFP targeted to the mitochondrial matrix (mitoGFP). Wide-field epifluorescene images are of body wall muscle cells from young adult animals. n, nucleus. Bar, 10 µm. (D) The average mitochondrial length in a given muscle cell was estimated using ImageJ (see Materials and Methods), and plotted as an individual point [wild-type n=16, ced-9(n2812lf); ced-3(n717lf)n=19, ced-9(n1950gf) n=14]; the mean value is indicated by a horizontal bar. (E) Mitochondrial ultrastructure in wild-type, ced-9(n2812lf); ced-3(n717lf) and ced-9(n1950sd) animals was examined in both body wall muscle and hypodermis by transmission electron microscopy. m, mitochondria. Bar, 500 nm.





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