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Figure 2


Fig. 2. Mitochondrial fission and fusion occur in the absence of CED-9. (A-F) Wild-type and dominant negative forms of DRP-1 were expressed using a myo-3 promoter in wild-type ced-9(+) and ced-9(n2812lf); ced-3(n717lf) animals. Similar mitochondrial morphologies were observed in both genetic backgrounds when body wall muscle cells were labeled with mitoGFP. n, nucleus. Bar, 10 µm. (G,H) Wild-type DRP-1 expressed using a myo-3 promoter in ced-9(n1950gf) animals resulted in a similar phenotype. (I) Increased DRP-1 expression induced mitochondrial fragmentation in wild-type, ced-9(n2812lf); ced-3(n717lf), and ced-9(n1950sd) backgrounds. Each point represents the proportion of cells with fragmented mitochondria from an independently generated transgenic line; the mean value is indicated by a horizontal bar, and error bars represent the standard error. The differences between wild-type and ced-9(n2812lf); ced-3(n717lf) backgrounds is significant, z=2.42, P<0.05; raw data is included as Table S1 in supplementary material. (J) Inactivating DRP-1 with dominant negative DRP-1(K40A) resulted in interconnected mitochondria in both wild-type and ced-9(n2812lf); ced-3(n717lf) backgrounds.





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