JCS035220 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Loss of wdb function induces apoptosis in the eye imaginal disc. Eye imaginal discs that are almost entirely mutant for wdb^IP (D-F; same genotype as in Fig. 4B) show increased staining with an antibody against activated caspase 3 (bright green spots throughout eye disc in D) compared with heterozygous siblings (A). Note that at this stage the mutant discs appear overgrown and do not show the organised cytoskeletal structures observed in differentiating cells of normal discs (compare F with C). Cleaved caspase 3 was detected using a rabbit antiserum from Cell Signalling (#9661) diluted at 1:1000. Scale bar: 20 µm.

• Supplemental Figure S2 -

  Fig. S2. Loss of wdb function leads to accumulation of pAkt1 specifically in nurse cell cytoplasm. Nurse cells mutant for wdb (non-GFP-stained) show elevated levels of pAkt1 (red) in the cytoplasm, but not in the DAPI-stained nucleus (blue). Scale bar: 50 µm.