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Fig. 4. Wdb is required for photoreceptor differentiation and survival in the developing eye. Eyflp-generated eye imaginal discs that are almost entirely homozygous for the wdb^IP mutation fail to differentiate adult eye structures (B), unlike discs from their heterozygous siblings (A). (C-V). Mosaic eyes generated in combination with a normal GFP-labelled third chromosome, were stained with phalloidin to detect the actin cytoskeleton (E,I,M,Q,U), and with two antibodies against the neuronal antigens 24B10 (D,H,L) and 22C10 (P,T), and wdb mutant clones identified by lack of GFP expression (C,G,K,O,S and merge in F,J,N,R,V). Arrows indicate position of mutant clones, which lack neuron-specific staining, and only show low levels of disorganised phalloidin staining. In the large clone shown in G-J, mutant tissue folds down and much of it is basal to the rest of the epithelium. For all eye discs, posterior is to the right. Scale bar: 20 µm.

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