spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Wdb physically interacts with Akt1 in Drosophila ovaries. Total protein extracts from wild-type whole larvae (640 µg) or adult ovaries (340 µg) were immunoprecipitated (IP) with antibodies against Wdb, pan-Akt1, pAkt1 or Flag epitope, resolved by SDS-PAGE and analysed by western blot (WB) using anti-Wdb and anti-pan-Akt1 antibodies. A faint signal is observed roughly in the location of Akt1 in the immunoprecipitation from larval extracts with Wdb antibody. However, Wdb is not immunoprecipitated from these extracts by anti-Akt1 antibodies. The proteins only show clear coimmunoprecipitation from ovary extracts. Arrows indicate the position of the Wdb protein doublet and Akt1 proteins. Asterisks indicate heavy IgG antibody chains recognised by the secondary antibodies. Molecular size markers are given on the left in kDa.





Right arrow Return to article