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Figure 1


Fig. 1. Quantification of siRNA-mediated pea3 and erm knockdown. (A,B) Relative mRNA expression levels of pea3 in si pea3 1-2 (A) and erm in si erm 1-2 (B) versus si ctrl transfected MMT cells, assessed by quantitative RT-PCR, 48 hours post transfection. Results are expressed as ratios of mRNA levels of pea3 or erm to cyclophilin (endogenous control standard) (1=si ctrl) and are the mean ± s.d. of three experiments in duplicate. (C) Western blot analysis of Pea3. Extracts of total protein of transfected MMT cells were analyzed using anti-Pea3 and anti-actin (control) antibodies 48 hours post transfection.





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