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Fig. 2. pea3 and erm knockdown impairs proliferation, migration and anchorage-independent growth ability of MMT cells. (A,B) Proliferation assays. si pea3 1-2 and si ctrl (A) or si erm 1-2 and si ctrl (B) transfected MMT cells were trypsinized and counted with a cell counter every day. Data are the mean ± s.d. of a representative experiment performed in triplicate. Experiments were reproduced four times in triplicate and gave the same results for each assay. (C) Migration assay. 24 hours post transfection, si pea3 1-2, si erm 1-2 or si ctrl transfected cells were seeded into the upper part of a Boyden chamber. After 18 hours, cells which have migrated through the membrane are counted. Experiments were done at least three times. Data are the mean ± s.d. of a representative experiment performed in triplicate. (D) Anchorage-independent growth assay. 24 hours post-transfection, si pea3 1-2, si erm 1-2 or si ctrl transfected cells were mixed with agar 0.65% and cultivated during 15 days. Experiments were done three times. Data are the mean ± s.d. of a representative experiment performed in triplicate.
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