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Figure 6


Fig. 6. Validation of the microarray experiments by semi- and quantitative RT-PCR. (A) mRNA expression levels of Pea3, Erm, Has2, Hgf, Ascl4, Fgfr1 and cyclophilin (endogenous control standard), in si ctrl (lane 1) versus si pea3 1 (lane 2) and si erm 2 (lane 3) transfected MMT cells, assessed by semi-quantitative RT-PCR. (B) Logarithmic fold-changes for pea3, erm, Fst, Suz12, Has2 and Stip1 genes as determined by quantitative RT-PCR for pea3 (black bars) and erm (gray bars) siRNA-mediated inhibition are shown. Results are expressed as ratios to cyclophilin mRNA levels. Error bars indicate ±s.e.m. of at least three independent biological replicates.





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