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Fig. 7. Effects of low-dose LatB on the activity of other actin nucleators. (A) Processive movement of EGFP-FRL1 was induced 40 seconds after 100 nM LatB perfusion. Arrowheads indicate FRL1 speckles moving processively. Owing to the decrease in speed, the speckles that moved in one direction for at least seven consecutive frames are labeled as processive after LatB treatment. Dotted lines indicate cell contour. The graph shows the number of EGFP-FRL1 speckles moving processively before and 50 seconds after treatment. Each colored line represents data from an individual cell. Data were analyzed using a two-tailed paired t-test; **P<0.01. (B) No obvious change in the density of speckles of the Arp2/3 complex was observed after 100 nM LatB treatment. Images were taken before and 90 seconds after treatment. The graph shows the number of single-molecule EGFP-p40 speckles before and 90 seconds after treatment (mean ± s.d., n=5 cells). The areas devoid of densely packed speckles were used for analysis. The loss of EGFP-p40 signal between the two conditions as a result of photobleaching is less than 10%. Scale bars: 2 µm.
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